| LIST OF CONTRIBUTORS |
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viii | (3) |
| FOREWORD |
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| Key Issues, Challenges and Future Opportunities in Gene Quantification |
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1 | (18) |
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| PART I: METHODS/TECHNOLOGY ISSUES |
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19 | (234) |
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A. GENE QUANTITATION BASED ON PCR AMPLIFICATION |
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19 | (150) |
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Present and Future Detection Formats for PCR Quantitation of Nucleic Acids |
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19 | (12) |
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Determination of Target Copy Number of Quantitative Standards Used in PCR Based Diagnostic Assays |
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31 | (14) |
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Quantification of Specific Nucleic Acids, Regulated RNA Processing and Genomic Polymorphisms Using Reversed-Phase HPLC |
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45 | (34) |
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Capillary Electrophoresis for Quantitative Genetic Analysis |
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79 | (18) |
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Quantitative PCR Technology |
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97 | (14) |
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Statistical Estimations of PCR Amplification Rates |
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111 | (18) |
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Fluorescence Monitoring of Rapid Cycle PCR for Quantification |
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129 | (16) |
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Kinetic Elisa-PCR: A Versatile Quantitative PCR Method |
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145 | (24) |
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B. GENE QUANTITATION BASED ON OTHER TARGET AMPLIFICATION SYSTEMS |
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169 | (36) |
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Quantitation of RNA by NASBA: Applications and Issues for HIV-1 and AIDS |
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169 | (20) |
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Application of Transcription-Mediated Amplification to Quantification of Gene Sequences |
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189 | (16) |
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C. GENE QUANTITATION BASED ON SIGNAL AMPLIFICATION |
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205 | (48) |
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Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Design and Performance |
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205 | (20) |
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Hybrid Capture^(TM) -- A Sensitive Signal Amplified Test for the Detection and Quantitation of Human Viral and Bacterial Pathogens |
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225 | (28) |
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| PART II: APPLICATIONS |
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253 | (116) |
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Quantification of Gene Expression by Competitive RT-PCR: The hCG(Beta)/LH(Beta) Gene Cluster |
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253 | (12) |
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Quantitative Detection of Mycoplasma DNA Using Competitive PCR |
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265 | (12) |
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The Detection and Quantification of bcr-abl in Chronic Myeloid Leukemia Following Marrow Transplantation |
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277 | (18) |
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Competitive RT-PCR Analysis of Brain Gene Expression During Inflammation and Disease |
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295 | (18) |
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Development and Application of Real-Time Quantitative PCR |
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313 | (14) |
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Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Research and Clinical Applications |
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327 | (16) |
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Quantification of Plasmid DNA Expression in Vivo |
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343 | (26) |
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| Index |
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369 | |
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