In continuous publication since 1994 and now in its sixth edition, Molecular Biotechnology: Principles and Applications of Recombinant DNA has been effective in introducing this complex field to students for more than 25 years. This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances.

This edition includes the latest techniques in DNA sequencing and genetic engineering of microbial, plant, and animal genomes, including human genome editing, as well as updates across many areas, such as:

• Immunological assays for disease diagnosis, more effective bacteriophage therapy, and new ways of dealing with antibiotic resistant bacteria
• New and developing vaccines for influenza, tuberculosis, and emerging viral threats, including Zika and SARS-CoV-2
• Engineering bacteria to perform plastic degradation and green algae to produce hydrogen, altering amino acid biosynthesis, and creating designer cellulosomes
• Production of humanized monoclonal antibodies in plants, modifying hybrid plants to produce clonal hybrids, and protecting plants from viral and fungal diseases

Molecular Biotechnology features nearly 600 detailed figures and is an ideal textbook for undergraduate and graduate courses in introductory biotechnology, as well as courses dedicated to utilizing this technology, such as medical, agricultural, environmental, and industrial biotechnology applications.

Bernard R. Glick, PhD, is a distinguished Professor Emeritus at the University of Waterloo, Ontario, Canada, where he was the chair of biology from 2002 to 2008. He has authored more than 400 publications that have garnered over 54,000 citations.

Cheryl L. Patten, PhD, is a Professor of microbiology in the Biology Department at the University of New Brunswick, Fredericton, Canada. She teaches courses in introductory biology, microbiology, and biotechnology.

Preface to the Sixth Edition

1 The Development of Molecular Biotechnology

2 Fundamental Technologies

3 Production of Recombinant Proteins

4 Molecular Diagnostics

5 Protein Therapeutics

6 Nucleic Acids as Therapeutic Agents

7 Vaccines

8 Industrial and Environmental Uses of Recombinant Microorganisms

9 Large-Scale Production of Proteins and Nucleic Acids from Recombinant Microorganisms

10 Genetic Engineering of Plants: Methodology

11 Transgenic Plants

12 Transgenic Animals

13 Molecular Biotechnology and Society

Amino Acids of Proteins and Their Designations

Index