Molecular Diagnosis of Infectious Diseases,9781588292216

Molecular Diagnosis of Infectious Diseases

by ; ; ;
Edition: 2nd
ISBN13: 9781588292216
ISBN10: 1588292215
Format: Hardcover
Pub. Date: 2003-12-01
Publisher(s): Humana Pr Inc
List Price: $213.98

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Summary

This second edition of a classic laboratory manual describes cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors apply these new methodologies successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, as well as to diagnostic reagents in immunological assays.

Table of Contents

Foreword v
Preface vii
Contributors xiii
Part I. Protein-Based Identification of Antigens
Proteomic Approaches to Antigen Discovery
3(16)
Karen M. Dobos
John S. Spencer
Ian M. Orme
John T. Belisle
Immunoproteomics
19(14)
Alexander Krah
Peter R. Jungblut
Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins
33(16)
Stephen Thompson
Part II. Dna-Based Identification of Antigens
Representational Difference Analysis of cDNA
49(18)
Lucas D. Bowler
Microarray Data Analysis and Mining
67(24)
Silvia Saviozzi
Giovanni Iazzetti
Enrico Caserta
Alessandro Guffanti
Raffaele A. Calogero
Expression Cloning
91(18)
Michael J. Lodes
Davin C. Dillon
Raymond L. Houghton
Yasir A. W. Skeiky
Part III. Identification of Epitopes and Immunomodulatory Components
Determination of Epitopes by Mass Spectrometry
109(12)
Christine Hager-Braun
Kenneth B. Tomer
Identification of T Cell Epitopes Using ELISpot and Peptide Pool Arrays
121(12)
Timothy W. Tobery
Michael J. Caulfield
Virus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte Activity
133(26)
Ludwig Deml
Jens Wild
Ralf Wagner
Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components
159(20)
Ludwig Deml
Michael Aigner
Alexander Eckhardt
Jochen Decker
Norbert Lehn
Wulf Schneider-Brachert
Part IV. Expression of Recombinant Proteins
High-Throughput Expression and Purification of 6xHis- Tagged Proteins in a 96-Well Format
179(12)
Jutta Dress
Jason Smith
Frank Schafer
Kerstin Steinert
Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins
191(6)
Markus Fuhrmann
Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA
197(16)
Marcus Graf
Ludwig Deml
Ralf Wagner
Part V. Purification, Modification, and Renaturation of Recombinant Proteins
Purification and immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluable Aggregates (Inclusion Bodies)
213(12)
Udo Reischl
Purification of Recombinant Protins with High Isoelectric Points
225(14)
Raffaele A. Calogero
Anna Aulicino
Refolding of Inclusion Body Proteins
239(16)
Marcus Mayer
Johannes Buchner
Small-Molecule-Protein Conjugation Procedures
255(14)
Stephen Thompson
Part VI. Characterization of Recombinant Proteins
Structural Characterization of Proteins and Peptides
269(30)
Rainer Deutzmann
Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors
299(22)
Claudia Hahnefeld
Stephan Drewianka
Friedrich W. Herberg
Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor
321(12)
Uwe Schaible
Michael Liss
Elke Prohaska
Jochen Decker
Karin Stadtherr
Hans Wolf
Part VII. Evaluation of Recombinant Proteins in Immunological Test Systems
Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
333(40)
John E. Butler
Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays
373(20)
Alan Warnes
Anthony R. Fooks
John R. Stephenson
Basic Problems of Serological Laboratory Diagnosis
393(36)
Walter Fierz
Molecular Diagnostics Resources on the Internet
429(18)
Larry Winger
Part VIII. Recombinant Receptor Molecules
Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
447(12)
Lars Toleikis
Olaf Broaders
Stefan Dubel
Index 459

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